Integrated Science – Air and Photosynthesis | F2 DBS (2026)

Living Things and Air

Integrated Science — Unit 7.1 Air and Unit 7.2 Photosynthesis

F2 DBS (2026)

01

Unit 7.1 — Air

Fill in the Blanks

Complete each statement using the most appropriate scientific term. More than one word may be used in a blank.

  1. Air is a __________ rather than a compound because its gases are not chemically combined.
  2. Nitrogen makes up approximately __________% of air, while oxygen makes up approximately __________%.
  3. The proportions of carbon dioxide and __________ in air may vary according to environmental conditions.
  4. Nitrogen is suitable for food packaging because it is relatively __________ and reduces contact between food and oxygen.
  5. A glowing splint __________ when it is inserted into a gas jar containing oxygen.
  6. When carbon dioxide is passed through limewater, an insoluble white substance called __________ is formed.
  7. Dry cobalt chloride paper changes from __________ to __________ in the presence of water.
  8. Solid carbon dioxide changes directly into gaseous carbon dioxide by a process called __________.
  9. Liquid nitrogen is useful for freezing food and preserving tissues because it has a very __________ boiling point.
  10. Carbon dioxide can be used in some fire extinguishers because it is __________ than air and does not support __________.
10 marks
02

Unit 7.1 — Air

True or False

State whether each statement is true or false. If it is false, rewrite the incorrect part to make the statement correct.

  1. Air is a compound containing chemically combined nitrogen and oxygen.
  2. The percentage of water vapour in air is the same in all places and at all times.
  3. The large amount of nitrogen in air dilutes oxygen and reduces the speed at which substances burn.
  4. Carbon dioxide makes up approximately 4% of normal atmospheric air.
  5. Both limewater and hydrogencarbonate indicator can be used to test for carbon dioxide.
  6. Oxygen burns brightly when a lighted splint is placed inside it.
  7. A gas that does not relight a glowing splint must be nitrogen.
  8. Dry cobalt chloride paper is pink before it is used to test for water.
  9. Carbon dioxide can be used in some fire extinguishers because it does not support the burning of ordinary materials.
  10. Pure oxygen can produce a hotter flame than air because air contains nitrogen that dilutes the oxygen.
10 marks
03

Unit 7.1 — Air

Composition and Identification of Gases

  1. A student states:
    “Air must be a compound because samples of air contain both nitrogen and oxygen.”
    1. State the approximate percentages of nitrogen and oxygen in air.
    2. Give two reasons why air is a mixture rather than a compound.
    3. Name one component of air whose proportion can vary considerably.
  2. Three gas jars P, Q and R contain oxygen, carbon dioxide and nitrogen, but their labels have been removed. The student is given a glowing splint and red hydrogencarbonate indicator. Describe a logical sequence of tests that would positively identify all three gases. Include the expected result at each stage.
  3. A glowing splint is inserted into an unknown gas and goes out. The student concludes that the gas must be carbon dioxide.
    1. Explain why the conclusion is not valid.
    2. Describe a further test that distinguishes carbon dioxide from nitrogen.
    3. State the expected result for each gas.
13 marks
04

Unit 7.1 — Air

Testing Carbon Dioxide and Combustion Products

  1. A gas is tested using limewater and red hydrogencarbonate indicator.
    1. State the positive result obtained using limewater.
    2. Name the insoluble substance responsible for the result.
    3. Write the word equation for the limewater test.
    4. State the colour change of hydrogencarbonate indicator.
    5. Explain why the indicator changes colour.
  2. A student places a glass funnel over a burning candle and draws the gases through two testing stations.
    Station 1 contains dry cobalt chloride paper.
    Station 2 contains limewater.
    1. State the colour change at Station 1.
    2. Name the substance detected at Station 1.
    3. State the observation at Station 2.
    4. Name the substance detected at Station 2.
    5. State two conclusions about the products of candle combustion.
  3. Equal volumes of fresh limewater are placed in two beakers.
    Beaker A: carbon dioxide from a soft drink is bubbled through it.
    Beaker B: the limewater is left exposed to classroom air.
    1. Explain why Beaker A becomes milky quickly.
    2. Explain why Beaker B does not become milky immediately.
    3. Predict what may form on its surface after several days.
15 marks
05

Unit 7.1 — Air

Uses and Properties of Common Gases

  1. A manufacturer is deciding whether to fill potato-chip bags with ordinary air, pure oxygen or pure nitrogen.
    1. Which gas should be selected?
    2. Explain why ordinary air is less suitable.
    3. Explain why pure oxygen is unsuitable.
    4. State the property of nitrogen that makes it suitable.
  2. Carbon dioxide can extinguish an ordinary paper fire, but it should not be used for some highly reactive metal fires.
    1. State two properties that make carbon dioxide suitable for extinguishing ordinary fires.
    2. Explain how these properties help to stop combustion.
    3. Explain why carbon dioxide may not extinguish burning magnesium.
  3. Solid carbon dioxide, or dry ice, is used to produce a low-lying stage fog.
    1. Name the change of state that occurs.
    2. State the approximate temperature at which dry ice changes into a gas.
    3. Explain why the carbon dioxide remains close to the floor.
    4. Explain why lying in a large amount of this fog could be dangerous.
15 marks
06

Unit 7.1 — Extension

Evaluating Experimental Evidence

This question introduces a slightly unfamiliar situation. Use the gas tests from the notes to interpret all the evidence.

A factory manager claims that the gas released from an exhaust pipe is pure nitrogen. A scientist collects a sample and obtains the following results.

Test Result
Glowing splint The splint does not relight.
Red hydrogencarbonate indicator The indicator slowly turns yellow.
Dry cobalt chloride paper The paper slowly turns pink.
  1. Does the glowing-splint result prove that the gas is nitrogen? Explain.
  2. What does the hydrogencarbonate-indicator result show?
  3. What does the cobalt chloride paper result show?
  4. Evaluate the manager’s claim using all three results.
7 marks
07

Unit 7.2 — Photosynthesis

Fill in the Blanks

Complete each statement using the most appropriate scientific term.

  1. Photosynthesis mainly takes place in the __________ of a plant.
  2. Green leaf cells contain structures called __________, in which the green pigment __________ is found.
  3. During photosynthesis, carbon dioxide and __________ are converted into glucose and oxygen.
  4. Photosynthesis converts __________ energy into chemical energy stored in __________.
  5. The gas released as a by-product of photosynthesis is __________.
  6. Plants can convert glucose into __________ for storage and __________ for making cell walls.
  7. Before investigating the factors necessary for photosynthesis, a plant should be kept in darkness for at least __________ hours.
  8. During the starch test, hot alcohol removes __________ from the leaf.
  9. After the alcohol treatment, the leaf is washed in hot water to remove the alcohol and __________ the leaf.
  10. Iodine solution changes from __________ to __________ when starch is present.
10 marks
08

Unit 7.2 — Photosynthesis

True or False

State whether each statement is true or false. If it is false, rewrite the incorrect part to make the statement correct.

  1. Photosynthesis mainly occurs in roots because roots absorb water.
  2. Chlorophyll absorbs the light energy needed for photosynthesis.
  3. Oxygen is the main food made by a plant during photosynthesis.
  4. Light energy is converted into chemical energy stored in glucose.
  5. Plants use all the glucose they make immediately in respiration.
  6. A plant is destarched by keeping it in darkness for at least 24 hours.
  7. Boiling a leaf in water removes its chlorophyll.
  8. Hot alcohol should be heated directly over a Bunsen flame so that chlorophyll can be removed quickly.
  9. The white parts of a variegated leaf normally do not contain chlorophyll.
  10. Finding starch in a leaf after an investigation provides evidence that photosynthesis has taken place.
10 marks
09

Unit 7.2 — Photosynthesis

Process, Energy and Uses of Glucose

  1. Write the word equation for photosynthesis.
  2. Identify:
    1. the two reactants;
    2. the main product;
    3. the by-product.
  3. State the energy conversion that occurs during photosynthesis.
  4. Explain the role of chlorophyll in photosynthesis.
  5. Explain how a plant can use glucose for each of the following:
    1. releasing energy;
    2. storing food;
    3. building cell walls;
    4. making proteins and chlorophyll.
12 marks
10

Unit 7.2 — Photosynthesis

Testing a Leaf for Starch

The four main stages of a starch test are shown below.

Stage Procedure
1 Boil the leaf in water.
2 Place the leaf in hot alcohol.
3 Wash the leaf in hot water.
4 Add iodine solution.
  1. State the purpose of each of the four stages.
  2. State:
    1. the positive result for starch;
    2. the negative result for starch.
  3. A student places a test tube of alcohol directly over a Bunsen flame.
    1. Explain why this is dangerous.
    2. Describe how the alcohol should be heated safely.
  4. Predict how forgetting to wash the leaf after the alcohol treatment may affect the leaf and the final observation.
12 marks
11

Unit 7.2 — Photosynthesis

Investigating the Need for Light

A student destarches a plant and covers part of one leaf on both sides with opaque black paper. The plant is then placed in bright light for eight hours.

  1. Explain why the black paper must cover both sides of the leaf.
  2. Identify:
    1. the independent variable;
    2. the dependent variable.
  3. Predict the iodine-test result for:
    1. the covered part of the leaf;
    2. the uncovered part of the leaf.
  4. State the conclusion of the investigation.
  5. Another student takes a plant directly from a sunny garden and carries out the same experiment without destarching it. Both the covered and uncovered regions turn blue-black.
    1. Explain why this result is unreliable.
    2. Explain why the covered region may still contain starch.
    3. Describe how the plant should have been prepared.
    4. Explain why keeping a plant in darkness removes its stored starch.
13 marks
12

Unit 7.2 — Photosynthesis

Chlorophyll and Carbon Dioxide

  1. A properly destarched variegated plant is placed in bright light. One of its leaves has green and white regions.
    1. Identify the independent variable.
    2. Predict the iodine-test result for the green regions.
    3. Predict the iodine-test result for the white regions.
    4. Explain the difference between the results.
    5. State the conclusion.
  2. Two similar, destarched plants are placed in sealed transparent bell jars.
    Jar A contains a chemical that absorbs carbon dioxide.
    Jar B contains an equal volume of water, which does not absorb carbon dioxide.
    Both jars receive the same light for the same length of time.
    1. Why are the plants destarched first?
    2. Identify the independent variable.
    3. State the purpose of Jar B.
    4. Name three control variables.
    5. Predict the starch-test result for a leaf from each plant.
    6. State the conclusion.
16 marks
13

Unit 7.2 — Extension

Measuring the Rate of Photosynthesis

This question introduces experimental ideas that are related to, but slightly beyond, the basic notes.

A student places an aquatic plant in water and positions a lamp at distances of 10 cm, 20 cm, 30 cm and 40 cm. The student counts the number of gas bubbles produced in five minutes at each distance.

  1. Identify:
    1. the independent variable;
    2. the dependent variable.
  2. Name the gas in the bubbles.
  3. Explain why each measurement should be repeated.
  4. State two variables that should be controlled.
  5. Explain why the lamp warming the water would make the investigation unfair.
  6. Give one limitation of counting bubbles as a method of measuring the volume of gas produced.
  7. The production of bubbles eventually stops increasing even when the lamp is moved closer. Suggest why adding more light may no longer increase the rate of photosynthesis.
10 marks
14

Unit 7.2 — Extension

Hydrogencarbonate Indicator and Water Plants

Four sealed boiling tubes contain red hydrogencarbonate indicator.

Tube Contents Condition
P Indicator only Bright light
Q Green water plant Bright light
R Green water plant Darkness
S Green water plant covered with black paper Bright light
Hydrogencarbonate indicator is:
  • yellow when the carbon dioxide concentration increases;
  • red at the normal carbon dioxide concentration;
  • purple when the carbon dioxide concentration decreases.
  1. Predict the final colour in Tube P.
  2. Predict the final colour in Tube Q and explain your answer.
  3. Predict the final colour in Tube R and explain your answer.
  4. Predict the final colour in Tube S and explain your answer.
  5. Explain why Tube P is necessary.
  6. A leaf from the plant in Tube Q turns blue-black after iodine solution is added. Explain how this result is connected to the indicator colour in Tube Q.
12 marks

Question 1

  1. mixture
  2. 78%; 21%
  3. water vapour
  4. unreactive
  5. relights
  6. calcium carbonate
  7. blue; pink
  8. sublimation
  9. low
  10. denser; burning or combustion

Question 2

  1. False. Air is a mixture because its component gases are not chemically combined.
  2. False. The percentage of water vapour varies with humidity, temperature and local conditions.
  3. True.
  4. False. Carbon dioxide makes up approximately 0.04% of atmospheric air.
  5. True.
  6. False. Oxygen supports burning, but oxygen itself does not burn in the splint test.
  7. False. Both nitrogen and carbon dioxide fail to relight a glowing splint, so a further test is needed.
  8. False. Dry cobalt chloride paper is blue and turns pink in the presence of water.
  9. True.
  10. True.

Question 3

    1. Air contains approximately 78% nitrogen and 21% oxygen.
    2. The gases in air are not chemically combined. The proportions of some components can vary, and the gases retain their own properties.
    3. Water vapour is an acceptable answer. Carbon dioxide may also vary.
  1. First insert a glowing splint into each jar. The gas that relights the glowing splint is oxygen. Add red hydrogencarbonate indicator to the remaining two gases. The gas that changes the indicator from red to yellow is carbon dioxide. The remaining gas is nitrogen. It is identified by deduction because it neither relights the glowing splint nor changes the indicator to yellow.
    1. The conclusion is invalid because both nitrogen and carbon dioxide fail to relight a glowing splint.
    2. Add red hydrogencarbonate indicator to the gas, or bubble the gas through the indicator.
    3. Carbon dioxide turns the indicator yellow. Nitrogen leaves the indicator red.

Question 4

    1. Limewater changes from colourless to milky or cloudy.
    2. The insoluble substance is calcium carbonate.
    3. Calcium hydroxide + carbon dioxide ⟶ calcium carbonate + water
    4. Hydrogencarbonate indicator changes from red to yellow.
    5. Carbon dioxide dissolves in the indicator and lowers its pH, making it more acidic.
    1. The cobalt chloride paper changes from blue to pink.
    2. Water or water vapour is detected.
    3. The limewater changes from colourless to milky or cloudy.
    4. Carbon dioxide is detected.
    5. Burning candle wax produces water vapour and carbon dioxide.
    1. The gas released from the soft drink contains a high concentration of carbon dioxide. It reacts quickly with the limewater to form calcium carbonate.
    2. Atmospheric air contains only about 0.04% carbon dioxide, so the reaction is too slow to produce an immediate visible change.
    3. A thin white crust, film or solid layer of calcium carbonate may eventually form on the surface.

Question 5

    1. The manufacturer should select pure nitrogen.
    2. Ordinary air contains about 21% oxygen. Oxygen can cause oxidation and make food spoil or become stale more quickly.
    3. Pure oxygen would increase oxidation and strongly support burning.
    4. Nitrogen is relatively unreactive.
    1. Carbon dioxide does not support the burning of ordinary materials and is denser than air.
    2. It sinks and forms a layer over the fire. This reduces the supply of oxygen required for combustion.
    3. Highly reactive metals such as magnesium may continue to burn by reacting with carbon dioxide.
    1. The process is sublimation.
    2. It occurs at approximately −78°C.
    3. Carbon dioxide is denser than air, so it remains near the floor.
    4. Carbon dioxide can collect near the floor and reduce the concentration of oxygen available for aerobic respiration. This could cause breathing difficulties or suffocation.

Question 6

  1. No. The result shows only that the gas is not oxygen. Both nitrogen and carbon dioxide fail to relight a glowing splint.
  2. The yellow indicator shows that carbon dioxide is present. Dissolved carbon dioxide lowers the pH of the indicator.
  3. The change from blue to pink shows that water or water vapour is present.
  4. The manager’s claim is false. The glowing-splint result is consistent with nitrogen but does not prove that the sample is nitrogen. The other two tests show that carbon dioxide and water vapour are present. Therefore, the exhaust is not pure nitrogen.

Question 7

  1. leaves
  2. chloroplasts; chlorophyll
  3. water
  4. light; glucose
  5. oxygen
  6. starch; cellulose
  7. 24
  8. chlorophyll
  9. soften
  10. brown; blue-black

Question 8

  1. False. Photosynthesis mainly occurs in green leaves.
  2. True.
  3. False. Glucose is the main food produced, while oxygen is a by-product.
  4. True.
  5. False. Glucose can also be stored as starch or used to make cellulose, proteins and chlorophyll.
  6. True.
  7. False. Boiling in water kills the leaf and damages its cell membranes. Hot alcohol removes chlorophyll.
  8. False. Alcohol is flammable and should be heated in a hot-water bath away from a naked flame.
  9. True.
  10. True.

Question 9

  1. Carbon dioxide + water ⟶ glucose + oxygen
    Light energy and chlorophyll are required.
    1. The reactants are carbon dioxide and water.
    2. The main product is glucose.
    3. The by-product is oxygen.
  2. Light energy is converted into chemical energy stored in glucose.
  3. Chlorophyll absorbs the light energy needed to drive photosynthesis.
    1. Glucose is used in respiration to release energy.
    2. Glucose is converted into starch for storage.
    3. Glucose is used to make cellulose for cell walls.
    4. Glucose is used to make other useful substances such as proteins and chlorophyll.

Question 10

    • Boil in water: kills the leaf and destroys or damages cell membranes so that alcohol and iodine can enter.
    • Heat in alcohol: removes chlorophyll so the iodine colour change can be observed clearly.
    • Wash in hot water: removes alcohol and softens the brittle leaf.
    • Add iodine solution: tests the leaf for starch.
    1. A blue-black colour is a positive result for starch.
    2. A brown or yellow-brown colour is a negative result.
    1. Alcohol is flammable and may catch fire when placed near a naked flame.
    2. The alcohol should be heated in a hot-water bath after the Bunsen flame has been turned off or moved safely away.
  1. The leaf may remain stiff and brittle because of the alcohol. It may break easily, and the iodine may not spread or penetrate properly, making the result difficult to observe.
Safety reminder: Never heat alcohol directly using a Bunsen flame.

Question 11

  1. The paper must cover both sides so that no light reaches the covered region.
    1. The independent variable is the presence or absence of light.
    2. The dependent variable is the presence of starch, shown by the iodine colour.
    1. The covered region remains brown because it receives no light and makes no new starch.
    2. The uncovered region turns blue-black because it receives light and produces starch.
  2. Light is necessary for photosynthesis and starch production.
    1. The plant may contain starch made before the investigation, so the test cannot show whether the starch was made during the experiment.
    2. The covered region may contain starch previously stored while the plant was in the sunny garden.
    3. The plant should have been kept in darkness for at least 24 hours before the investigation.
    4. Photosynthesis stops in darkness, but respiration continues. Stored starch is converted into soluble substances and used in respiration.

Question 12

    1. The independent variable is the presence or absence of chlorophyll.
    2. The green regions turn blue-black.
    3. The white regions remain brown.
    4. Green regions contain chlorophyll, absorb light and carry out photosynthesis. White regions lack chlorophyll and do not produce glucose or starch.
    5. Chlorophyll is necessary for photosynthesis.
    1. Destarching removes previously stored starch so that any starch found was made during the investigation.
    2. The independent variable is the availability of carbon dioxide.
    3. Jar B is the control. It shows that a similar plant can produce starch under the same conditions when carbon dioxide is available.
    4. Any three of: light intensity, temperature, plant species or size, water supply, duration of the experiment and size of the bell jar.
    5. The leaf from Jar A remains brown. The leaf from Jar B turns blue-black.
    6. Carbon dioxide is necessary for photosynthesis.

Question 13

    1. The independent variable is the distance between the lamp and the plant, which changes the light intensity.
    2. The dependent variable is the number of bubbles produced in five minutes.
  1. The gas is oxygen.
  2. Repeating the measurements helps identify unusual results, improves reliability and allows a mean to be calculated.
  3. Any two of: temperature, type or size of plant, duration of counting, amount of water and availability of carbon dioxide.
  4. Temperature also affects biological reactions. If the water becomes warmer, the student cannot be certain whether the change in rate was caused by light intensity or temperature.
  5. Bubbles may have different sizes, so the number of bubbles is not an accurate measurement of the volume of oxygen.
  6. Another necessary factor may have become limiting. For example, the plant may use up the available carbon dioxide. Adding more light cannot increase photosynthesis if there is insufficient carbon dioxide.

Question 14

  1. Tube P remains red. There is no organism to change the carbon dioxide concentration.
  2. Tube Q turns purple. In bright light, photosynthesis removes carbon dioxide faster than respiration releases it.
  3. Tube R turns yellow. In darkness, photosynthesis cannot occur, but respiration continues and releases carbon dioxide.
  4. Tube S turns yellow. The black paper prevents light from reaching the plant, so photosynthesis stops while respiration continues.
  5. Tube P is a control. It shows that bright light alone does not change the colour of hydrogencarbonate indicator.
  6. The blue-black iodine result shows that starch was produced, so photosynthesis occurred. During photosynthesis, the plant used carbon dioxide, reducing its concentration and causing the indicator to turn purple.
Key connection: Photosynthesis removes carbon dioxide and produces glucose, which may then be converted into starch.
Scroll to Top